Effect of cycloheximide on the infective yield of a genital strain of Chlamydia trachomatis in McCoy cells.
نویسندگان
چکیده
The yield of infectious progeny of a genital strain of Chlamydia trachomatis in cycloheximide-treated McCoy cell cultures was 11-fold lower than that in untreated monolayers.
منابع مشابه
Amino acid requirements of a Chlamydia trachomatis genital strain in McCoy cell cultures.
Amino acid requirements of a Chlamydia trachomatis were defined in McCoy cell monolayer cultures, depleted of their nutrient reserves by maintenance in Earle balanced salt solution for at least 2 days, and then treated at infection with 1 microgram of cycloheximide per ml, an inhibitor of host cell protein and deoxyribonucleic acid synthesis. Serum-free Waymouth MB 752/1 medium lacking one amin...
متن کاملImproved method for isolation and growth of Chlamydia trachomatis in McCoy cells treated with cycloheximide using polyethylene glycol.
The sensitivity of non-replicating McCoy cells pretreated with polyethylene glycol for the isolation of Chlamydia trachomatis from clinical specimens and for the growth of a laboratory strain was compared with the sensitivity of untreated non-replicating cell cultures. The concentration of polyethylene glycol in different solutions and the time of addition to the cell culture medium were critic...
متن کاملComparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.
McCoy cells treated in six different ways, in addition to untreated cells, were compared to determine which gave rise to the largest number of Chlamydia trachomatis inclusions when tested with a laboratory-passaged strain. The same batch of cells was treated by irradiation, preinoculation exposure to cytochalasin B or 5-iodo-2'-deoxyuridine, and postinoculation exposure to cycloheximide, hydroc...
متن کاملCultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.
An isolation technique for Chlamydia trachomatis using McCoy cells is described. In contrast to earlier techniques employing such cells, no pretreatment of the cells was used. The glutarimide antibiotic cycloheximide was added to the culture medium used for incubating the cells after infection. Cycloheximide was used at concentrations that depressed, but did not completely inhibit, the metaboli...
متن کاملP-59: Chlamydia Trachomatis Infection in Femaleswith Secondary Infertility
Background: We designed a prospective study to assess the role of Chlamydia in secondary infertility Materials and Methods: 40 women with secondary infertility and 30 term healthy pregnant women of similar age composition were studied for past and present chlamydia trachomatis infection. Women attending the outpatient department of Obstetrics and Gynaecology with complaint of secondary infertil...
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عنوان ژورنال:
- Infection and immunity
دوره 33 1 شماره
صفحات -
تاریخ انتشار 1981